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USA: As an immediate follow-on to the Virtual International Water Research Summit on Environmental Surveillance of COVID-19 Indicators in Sewersheds, The Water Research Foundation will release Requests for Qualifications (RFQs) and seek funding partners to accelerate research in three key areas:

Interlaboratory and Methods Assessment
Currently, no standard methods have been established for the detection of the genetic fingerprint of COVID-19 in water or wastewater. While environmental surveillance of sewer-sheds holds significant promise as a near-term indicator of community-level trends of COVID-19, this information will be most useful for public health decision-makers if it can be repeated and compared across different laboratories that are analyzing samples. This research will engage several laboratories to determine:

  • Which methods produce the most reliable results for analyzing the genetic fingerprint of COVID-19
  • The extent to which laboratories are able to reproduce sample results

Stability of Genetic Signal in Wastewater Matrix
This research seeks to identify potential changes in the genetic fingerprint of COVID-19 as it travels through sewer systems. This research will address:

  • Dilution of the genetic signal in sewage
  • Loss of the genetic fingerprint while in transit in the collection system due to adsorption and degradation
  • Loss of the genetic fingerprint due to interference from other wastewater constituents
  • Effect of the wastewater treatment process on the genetic signal

Impact of Storage and Pre-Treatment Methods on Signal Strength
Several pretreatment methods have been utilized to protect wastewater workers or to preserve samples for future analysis. The objective of this research will be to identify the effect of the following pre-treatments on genetic fingerprint strength:

  • Heating wastewater samples to inactivate potentially live virus
  • Addition of chemicals to inactivate potentially viable pathogens in the wastewater samples
  • Storage of samples at 4°C, -20°C, -70°C, -80°C.

The Water Research Foundation is actively seeking partners to leverage our investment in this research, and we anticipate posting the first RFQ in the coming weeks.

The Water Research Foundation (WRF) is the world’s leading research organization advancing the science of all water to meet the evolving needs of its subscribers and the water sector. WRF is a nonprofit, educational organization that funds, manages, and publishes research on the technology, operation, and management of drinking water, wastewater, reuse, and stormwater systems – all in pursuit of protection of public health and the environment. WRF represents approximately 1,200 subscribers, hosts an online research library of more than 2,300 completed projects valued at USD 700 million, manages an innovation platform (LIFT Link) with a database of more than 140 innovative technologies, and supports the world’s largest body of stormwater best practice data.

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